To get Researchers, using techniques like flow cytometry, Western Blotting, ELISA and immunohistochemistry, antibodies are being used to quantify antigens in complex biological selections. These days, it is possible to measure hundreds of antigens simultaneously with multiplex immunoassay technologies. Generally all of these antibody-based detection techniques require a label (antibody labelling) of some description that enables measurability. lamin B1 antibody
The vast majority of antibodies that are offered commercially are not labelled, this means they are not quantifiable. Generally only a tiny number of antibodies, those deemed commercial valuable by the antibody manufacturers, are available in conjugated/labelled form. Also, for the antibodies available in conjugated form, it is generally for a tiny range labels. This can be very restricting in regards to experimental design.
Using unlabeled antibodies comes with several inherent problems particularly with multiplex assays. For Roundabout detection, which commonly runs on the secondary antibody with the necessary label, it is difficult to create a snowboard of secondary reagents with the desired selectivity and not enough unwanted cross reactions. These problems are get over, however, by covalently connecting the label straight to the primary antibody, reducing the complexity of immunoassays and quickly and simply producing a flow cytometry antibody ready for analysis.
Traditionally, antibody labelling has included chemical modification and has been conducted by commercial organisations with expert understanding of the required techniques. Nevertheless , due to significant advances in antibody labels technologies, this once difficult, frustrating and costly method quickly performed by anyone in the lab without the need for specialist training or experience.